NAD+ (β-Nicotinamide Adenine Dinucleotide)
Not a peptide — a dinucleotide cofactor, CAS 53-84-9, 663.43 Da. It belongs in this catalogue because it is bought alongside them, but it is purified and quantified by completely different chemistry.
Pricing and pack sizes
| Size | Cat. no. | Pack | Price per kit | Per vial |
|---|---|---|---|---|
| 100 mg | NJ100 | 10 vials | $35.00 | $3.50 |
| 250 mg | NJ250 | 10 vials | $50.00 | $5.00 |
| 500 mg | NJ500 | 10 vials | $75.00 | $7.50 |
| 1000 mg | NJ1000 | 10 vials | $125.00 | $12.50 |
Prices are in USD and are per kit of 10 vials — not per vial. Larger quantities and mixed kits are quoted on request.
Monograph
| Compound | β-Nicotinamide adenine dinucleotide, free acid (NAD+) |
|---|---|
| CAS Registry Number | 53-84-9 |
| Molecular Formula | C21H27N7O14P2 |
| Molecular Weight | 663.43 g/mol |
| Class | Dinucleotide cofactor — not a peptide |
| Purity | ≥ 99.0% (RP-HPLC, UV 260 nm), NADH resolved |
| Identity Confirmation | ESI-MS in negative mode; A260/A280 ratio reported |
| Available pack sizes | 100 mg, 250 mg, 500 mg, 1000 mg — 10 vials per kit |
| Appearance | White to pale yellow powder |
| Solubility | Freely soluble in water |
| Storage | −20 °C, desiccated, protected from light and moisture |
| Documentation | Lot-specific COA with HPLC chromatogram and MS spectrum |
What NAD+ is, and why it is characterised differently
NAD+ is a dinucleotide: two nucleotides joined through a phosphoanhydride linkage, carrying an adenine and a nicotinamide ring. It is the central redox carrier in cellular metabolism and the substrate for sirtuins, PARPs and CD38 — which is why it is studied in far more contexts than any peptide on this site.
Two practical consequences for analytical work:
- Detection at 260 nm, not 220 nm. The adenine ring absorbs strongly at 260 nm. Reporting "HPLC purity at 220 nm" for NAD+ means the detection point was chosen for peptides, not for this molecule.
- NADH must be resolved, not ignored. The reduced form differs by two hydrogen atoms (mass +2) and co-elutes with NAD+ on many generic gradients. A method that does not separate them reports a purity figure that includes the oxidised/reduced mixture.
NAD+ is also hygroscopic and degrades in alkaline solution and on prolonged light exposure. Water content and storage form matter as much as the purity number.
Research context
Summarises mechanisms reported in the literature, for scientific orientation only. Not a description of any approved or established clinical application.
Redox cofactor
NAD+ accepts electrons in glycolysis, the TCA cycle and β-oxidation, and donates them to the electron transport chain. This is textbook biochemistry and does not depend on any therapeutic claim.
Signalling substrate
Beyond redox, NAD+ is consumed by sirtuins (deacetylation), PARPs (DNA repair) and CD38 (calcium signalling). The same molecule therefore sits at the centre of several distinct research fields, which is why demand is broad.
Note on evidence status
NAD+ is not an approved medicine. Oral supplementation is marketed heavily and studied unevenly; the material here is an analytical reference standard, not a supplement for consumption.
Frequently asked questions
Is NAD+ a peptide?
No. It is a dinucleotide — two nucleotides linked by a phosphoanhydride bond — with no amide backbone and no residue sequence.
Why is purity measured at 260 nm?
The adenine ring absorbs strongly at 260 nm. The 220 nm wavelength used for peptides is the wrong detection point for a nucleotide.
Do you resolve NADH from NAD+?
Yes. NADH differs by two hydrogen atoms and co-elutes with NAD+ on many generic gradients. Our method resolves them and the chromatogram is on the COA.
What pack sizes are available?
100 mg, 250 mg, 500 mg and 1000 mg vials, supplied as 10 vials per kit. Prices per kit are listed on this page.
How should NAD+ be stored?
−20 °C, desiccated, protected from light. The material is hygroscopic and degrades in alkaline solution, so keep it dry and avoid repeated freeze–thaw of solutions.
Is NAD+ approved for human use?
Not as a medicine. It is sold as a supplement in some markets, but the material here is an analytical reference standard and is not for human consumption.
Research use only
This product is a chemical reference standard intended exclusively for in-vitro laboratory research and analytical method development. It is not a pharmaceutical, drug, food, food additive, cosmetic, supplement, or veterinary product, and it must not be administered to humans or animals.